產(chǎn)品詳情介紹
CD45是一種180-240 kDa的單鏈 I 型跨膜糖蛋白[1]，又稱Protein tyrosine phosphatase, receptor type C（PTPRC），或T200 glycoprotein、T200 leukocyte common antigen 等，在除紅細(xì)胞或血小板外的所有造血細(xì)胞的質(zhì)膜上均有表達(dá)[2]。CD45結(jié)構(gòu)包括一個(gè)胞外結(jié)構(gòu)域、一個(gè)跨膜區(qū)和兩個(gè)串聯(lián)胞內(nèi)催化結(jié)構(gòu)域。CD45是protein tyrosine phosphatase（PTP）家族成員，能夠調(diào)節(jié)細(xì)胞生長(zhǎng)、分化、細(xì)胞周期和癌基因轉(zhuǎn)化等多種細(xì)胞過(guò)程[3]。另外，CD45還在T細(xì)胞和B細(xì)胞抗原受體介導(dǎo)的去磷酸化底物（包括p56lck、p59fyn和其他src家族激酶）激活中起著關(guān)鍵作用[4]。本產(chǎn)品為小鼠抗人CD45單克隆抗體，用于WB、IF和FC方法特異性檢測(cè)人CD45蛋白。
WB: ProteinFind? Anti-CD45 Mouse Monoclonal Antibody 檢測(cè) Jurkat 細(xì)胞中 CD45 蛋白的表達(dá)。預(yù)測(cè)分子量：147 KDa,實(shí)際分子量：200 KDa,一抗稀釋倍數(shù)：1:1000
IF: ProteinFind? Anti-CD45 Mouse Monoclonal Antibody (綠色)檢測(cè) Jurkat 細(xì)胞 CD45 的定位，Hoechst 用于標(biāo)記細(xì)胞核(藍(lán)色)。一抗稀釋倍數(shù):1:100
FC: ProteinFind? Anti-CD45 Mouse Monoclonal Antibody (綠色)對(duì)人外周血(A圖)及 Jurkat 細(xì)胞(B圖)的 FC 檢測(cè)結(jié)果。陰性對(duì)照為 Mouse IgG1 Isotype Control (紅色)。一抗稀釋倍數(shù):1: 100
CD系列產(chǎn)品介紹視頻
[1] Charbonneau H, Tonks NK, Walsh KA, et al. The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase [J]. Proc Natl Acad Sci U S A. 1988, 85(19): 7182-6.
[2] Nakano A, Harada T, Morikawa S, et al. Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines [J]. Acta Pathol Jpn. 1990, 40(2): 107-15.
[3] Trowbridge IS, Johnson P, Ostergaard H, et al. Structure and function of CD45: a leukocyte-specific protein tyrosine phosphatase [J]. Adv Exp Med Biol. 1992, 323: 29-37.
[4] Penninger JM, Wallace VA, Kishihara K, et al. The role of p56lck and p59fyn tyrosine kinases and CD45 protein tyrosine phosphatase in T-cell development and clonal selection [J]. Immunol Rev. 1993, 135: 183-214.
